Skeletal development and repair : methods and protocols

상세정보

자료유형	단행본
서명/저자사항	Skeletal development and repair : methods and protocols / edited by Matthew J. Hilton.
개인저자	Hilton, Matthew J.
발행사항	New York : Humana Press : Springer, 2014.
형태사항	x, 328 p. : ill. (some col.) ; 27 cm.
총서사항	Methods in Molecular Biology,1064-3745 ; 1130 Springer protocols
ISBN	9781627039888 : 9781627039895 (electronic bk.) 1627039899 (electronic bk.)
서지주기	Includes bibliographical references and index.
내용주기	Overview of Skeletal Development -- Overview of Skeletal Repair (Fracture Healing and Its Assessment) -- Generation of Closed Transverse Fractures in Small Animals -- The Murine Femoral Bone Graft Model and a Semi-Automated Histomorphometric Analysis Tool -- Surgical Induction of Posttraumatic Osteoarthirits -- A Mouse Model of Flexor Tendon Repair -- Cartilage Explant Cultures -- Renal Capsule Transplantations to Assay Skeletal Angiogenesis -- Whole Mount Skeletal Staining -- Tips and Techniques for Processing and Sectioning Undecalcified Murine Bone Specimens -- Preparation of Thin Sections from Non-fixed and Undecalcified Hard Tissues Using Kawamoto's Film Method (2012) -- Demineralized Murine Skeletal Histology -- Beta-Galactosidase Staining in the Skeleton -- Whole-Mount In Situ Hybridization on Murine Skeletogenic Tissues -- Non-Radioactive In Situ Hybridization on Skeletal Tissue Sections -- Radioactive In Situ Hybridization to Detect Gene Expression in Skeletal Tissue Sections -- Proliferation Assays (BrdU and EdU) on Skeletal Tissue Sections -- TUNEL Assay on Skeletal Tissue Sections to Detect Apoptosis -- Analysis of Chondrogenesis Using Micromass Cultures of Limb Mesenchyme -- Isolation and Culture of Murine Primary Chondrocytes -- Bone Marrow Stromal Cell Assays : In Vitro and In Vivo -- Isolation and Culture of Neonatal Mouse Calvarial Osteoblasts -- RANKL-Based Osteoclastogenic Assays from Murine Bone Marrow Cells -- Hematopoietic Stem Cell Cultures and Assays.
일반주제명	Skeleton --Laboratory manuals. Bones --Growth --Laboratory manuals. Mice as laboratory animals.
분류기호(DDC)	611.71
언어	영어